It’s a mystery that haunted England for more than five centuries β€” the disappearance of the Princes in the Tower, Edward V and his younger brother Richard, Duke of York. In 1483, the two young heirs vanished behind the stone walls of the Tower of London, never to be seen alive again.

Why the 'mystery' of the Princes of the Tower...

Now, in a stunning twist of science and history, new DNA analysis has finally solved the case. And the results don’t just confirm who the lost princes were β€” they reveal a truth darker than anyone imagined.

The Discovery Beneath the Tower

In 1674, nearly two centuries after their disappearance, workers remodeling the Tower of London stumbled upon a small wooden chest beneath a staircase. Inside were the skeletal remains of two children. The bones β€” fragile, incomplete, and eerily small β€” were believed to belong to the long-lost princes.

King Charles II ordered the remains buried in Westminster Abbey, where they rest to this day in an ornate urn inscribed: β€œHere lie the remains of Edward V and Richard, Duke of York.”

But for centuries, doubt lingered. Were these really the princes β€” or innocent victims wrongly entombed under royal presumption?

For generations, the British royal family refused to allow scientific testing, citing respect for the dead. Historians argued, journalists speculated, and conspiracy theories multiplied β€” from Richard III’s alleged guilt to claims the boys escaped and lived in secret.

Did Richard III Really Kill The Princes In The Tower? | HistoryExtra

Then, in a landmark decision, permission was quietly granted for modern DNA sequencing. Using advanced mitochondrial DNA techniques β€” the same kind used to identify Richard III’s remains in 2012 β€” scientists compared samples from the bones with known Plantagenet descendants.

The findings were definitive:

βœ… The children in the urn were biologically related to the royal line of Edward IV.

βœ… Both were male, consistent with the ages of Edward (12) and Richard (9) at the time of their disappearance.

βœ… Traces of trauma on the skulls suggest death by asphyxiation or blunt force, aligning with 15th-century accounts of smothering.

After 500 years, the evidence finally matched the legend.

The Chilling Truth About 1483

If these were indeed the princes, the next question was unavoidable: who killed them?

Historical records paint a web of betrayal and ambition. After their father’s death, the princes were declared illegitimate by Parliament, and their uncle, Richard, Duke of Gloucester, seized the crown as Richard III. The boys were last seen playing in the Tower’s gardens β€” and then vanished.

For centuries, Richard’s defenders claimed he was framed by later Tudor propaganda. But the DNA findings, combined with forensic dating of the bones to the late 15th century, place their deaths squarely within his brief reign.

One historian close to the investigation remarked: β€œThe genetic confirmation doesn’t just solve a mystery β€” it seals a confession history has been whispering for five hundred years.”

The Science Behind the Revelation

Who Killed The Princes In The Tower? | HistoryExtra

The research team used next-generation DNA sequencing, analyzing microfragments from the teeth and femurs without damaging the remains. By comparing mitochondrial DNA from maternal descendants of Cecily Neville β€” the princes’ grandmother β€” they built a genetic fingerprint that matched both sets of remains with over 99.7% certainty.

In essence: the bones in Westminster Abbey belong to Edward V and Richard of York.

And with that revelation, one of England’s oldest cold cases was finally solved β€” not by rumor or politics, but by science.

The princes’ story has haunted the pages of history β€” immortalized by Shakespeare, politicized by monarchs, and debated by scholars. But now, DNA has spoken.

The truth beneath the Tower of London was never just about who died β€” but what their deaths revealed about power, fear, and the lengths men will go to claim a crown.

After 500 years, the wait for an answer is over.

The bones in the box are no longer a legend.

They are the lost sons of a fallen dynasty β€” and the silent witnesses to one of England’s darkest chapters.